human hepatic cell line hepg2  (ATCC)

Journal: Medicina

Article Title: Retrospective Analysis of a Quince, Olive Leaf, and Amaranth Nutraceutical in Patients with Metabolic Syndrome

doi: 10.3390/medicina61091638

Figure Lengend Snippet: Preclinical evaluation of QUINOLAM on metabolic and cellular endpoints in HepG2 cells. ( A ) Cell viability assessed by MTT assay after 24 h exposure to increasing concentrations of QUINOLAM (0.003 to 1.6 mg/mL). No cytotoxic effects were observed up to 1.6 mg/mL. SDS (1 mg/mL) was used as a positive control. ( B ) Glucose uptake measured using the 2-NBDG fluorescent analog following 24 h treatment with QUINOLAM at 0.5, 1.0, and 2.5 mg/mL. A dose-dependent increase in glucose uptake was observed, with significant enhancement at 2.5 mg/mL ( p < 0.01 vs. control). ( C ) LDL receptor (LDL-R) protein expression determined by ELISA after 24 h exposure to QUINOLAM. Treatments with 1.0 and 2.5 mg/mL significantly upregulated LDL-R levels compared to untreated controls ( p < 0.05). ( D ) Antioxidant activity of QUINOLAM evaluated using the Trolox Equivalent Antioxidant Capacity (TEAC) assay. QUINOLAM displayed strong antioxidant potential in a dose-dependent manner, with significant increases in TEAC values at 0.1 and 0.2 mg/mL (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. control). All data are expressed as mean ± standard deviation from three independent experiments.

Article Snippet: All in vitro experiments were conducted using the HepG2 human hepatic cell line (ATCC, Manassas, VA, USA).

Techniques: MTT Assay, Positive Control, Control, Expressing, Enzyme-linked Immunosorbent Assay, Antioxidant Activity Assay, Standard Deviation

Journal: Plants (Basel, Switzerland)

Article Title: Hydroethanolic Extract of Polygonum aviculare L. Mediates the Anti-Inflammatory Activity in RAW 264.7 Murine Macrophages Through Induction of Heme Oxygenase-1 and Inhibition of Inducible Nitric Oxide Synthase.

doi: 10.3390/plants13233314

Figure Lengend Snippet: Figure 4. Antioxidant effects of PAL hydroethanolic extract by activation of Nrf2 signaling path- way in RAW 264.7 macrophages. Protein levels of (A) nuclear Nrf2 and (B) cytoplasmic HO-1 in RAW 264.7 macrophages were quantitatively analyzed. (C) ARE activity by PAL hydroethanolic extract in HepG2-ARE cells. (D) Intracellular ROS level by PAL hydroethanolic extract in RAW 264.7 macrophages. Data are presented as the mean ± SEM (N = 3). A statistical significance com- pared with control group at p < 0.05 and p < 0.01 was marked by an asterisk (*) and double asterisk (**), respectively. SFN, sulforaphane; tBHP, tert-butyl hydroperoxide; tBHQ, tertiary-butylhydroquinone.

Article Snippet: HepG2 human hepatic cells harboring the construct containing the antioxidant response element (ARE) sequence following the luciferase-encoding gene were purchased from BPS Bioscience (#60513).

Techniques: Activation Assay, Activity Assay, Control